Overview

  • Product name
    Monkey IL-10 ELISA Kit (Interleukin-10)
    See all IL10 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6 < 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 6 < 10%
  • Sample type
    Cell culture supernatant, Serum
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.3 U/ml
  • Range
    3.9 U/ml - 250 U/ml
  • Recovery

    95 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Monkey
  • Product overview

    Abcam’s IL-10 (Interleukin-10) Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Monkey IL-10 in cell culture supernatant and serum (baboon, cynomolgus).

    This assay employs an antibody specific for Monkey IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Monkey IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Standard is recombinant protein produced in E.coli.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119530.

Protocols

References

ab119530 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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