Monkey IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab119533)


  • Product nameMonkey IL-12 p70 ELISA Kit (Interleukin-12 p70)
    See all IL12 p70 kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall < 5%
    Sample n Mean SD CV%
    Overall 4.8%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    2.6 U/ml
  • Range
    3.9 U/ml - 250 U/ml
  • Recovery

    105 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Monkey
  • Product overview

    Abcam’s IL-12 p70 (Interleukin-12 p70) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey IL-12 p70 concentrations in Cell culture supernatant and serum (baboon).

    Monkey IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Monkey IL-12 p70 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Monkey IL-12 p70 captured on the plate.

  • Notes

    Standard is recombinant protein produced in E.coli.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionCytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • CLMF
    • CLMF p35
    • Cytotoxic lymphocyte maturation factor 1
    • Cytotoxic lymphocyte maturation factor 1 p35
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • IL-12 subunit p35
    • IL-12A
    • IL12A
    • IL12A_HUMAN
    • IL35 subunit
    • Interleukin 12 alpha chain
    • Interleukin 12 p35
    • Interleukin 12 p70
    • Interleukin-12 subunit alpha
    • Natural killer cell stimulatory factor 1
    • Natural killer cell stimulatory factor 1 35 kD subunit
    • NF cell stimulatory factor chain 1
    • NFSK
    • NK cell stimulatory factor chain 1
    • NKSF1
    • P35
    see all


Our Abpromise guarantee covers the use of ab119533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Monkey IL-12 p70 ELISA Kit (Interleukin-12 p70) images

  • Representative Standard Curve using ab119533.



References for Monkey IL-12 p70 ELISA Kit (Interleukin-12 p70) (ab119533)

ab119533 has not yet been referenced specifically in any publications.

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Thank you very much for contacting us. Please find below the response from the lab:

We have not tested our ELISA in high salt Tris-based buffers and thus cannot tell you with a certainty how this buffer will affect the assay.

High sal...

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