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|Sample type||Average %||Range|
|Serum||88||72% - 125%|
Abcam’s IL-8 (Interleukin-8) Monkey ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of monkey IL-8 in culture supernatant and serum (cynomolgus, baboon).
IL-8 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a biotin-conjugated anti-monkey IL-8 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-8 captured on the plate.
Standard is recombinant protein produced in E.coli.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-monkey IL8 polyclonal antibody||1 x 70µl|
|Microplate coated with monoclonal antibody to monkey IL-8 (12 x 8 wells)||1 unit|
|Monkey IL-8 Standard Concentrate (125,000 U/mL)||2 x 100µl|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119550 has not yet been referenced specifically in any publications.