Overview

  • Product nameMonkey IL2 ELISA Kit (Interleukin-2)
    See all IL2 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 < 7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 < 5%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    10 U/ml
  • Range
    20 U/ml - 1250 U/ml
  • Recovery

    80 %

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Monkey
  • Product overview

    Abcam’s IL-2 (Interleukin-2) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey IL-2 (Interleukin-2)concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, urine and whole blood.

    IL-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-2 captured on the plate.

  • Notes

    Standard is recombinant protein produced in E.coli.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionProduced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • Involvement in diseaseNote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • Sequence similaritiesBelongs to the IL-2 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Aldesleukin
    • IL 2
    • IL-2
    • IL2
    • IL2_HUMAN
    • Interleukin 2
    • Interleukin-2
    • Interleukin2
    • Involved in regulation of T cell clonal expansion
    • Lymphokine
    • T cell growth factor
    • T-cell growth factor
    • TCGF
    see all

Applications

Our Abpromise guarantee covers the use of ab119541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Monkey IL2 ELISA Kit (Interleukin-2) images

  • Representative Standard Curve using ab119541.

Protocols

References for Monkey IL2 ELISA Kit (Interleukin-2) (ab119541)

ab119541 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"