Abcam’s IL-2 (Interleukin-2) Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey IL-2 (Interleukin-2)concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, urine and whole blood.
IL-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-2 captured on the plate.
Standard is recombinant protein produced in E.coli.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-monkey IL-2 polyclonal antibody||1 x 70µl|
|Microplate coated with monoclonal antibody to monkey IL-2||1 unit|
|Monkey IL-2 Standard lyophilized (2.4 U/ml upon reconstitution)||2 vials|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119541 has not yet been referenced specifically in any publications.