Abcam’s Soluble TNF Receptor I Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey Soluble TNF Receptor I concentrations in Cell culture supernatant and serum (baboon, cynomolgus).
Soluble TNF Receptor I specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated Soluble TNF Receptor I detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is then added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the Soluble TNF Receptor I amount of sample captured in plate.
Standard is recombinant protein produced in E.coli.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||2 units|
|HRP-Conjugate anti-Monkey Soluble TNF Receptor I monoclonal antibody||1 x 200µl|
|Microplate coated with monoclonal antibody to monkey soluble TNF Receptor I (12 x 8 wells)||1 unit|
|Soluble TNF Receptor I Standard||2 x 50µl|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119562 has not yet been referenced specifically in any publications.