Overview

  • Product name
    Monkey Soluble TNF Receptor I ELISA Kit
    See all TNF Receptor I kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4 2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4 9%
  • Sample type
    Cell culture supernatant, Serum
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.06 U/ml
  • Range
    0.09 U/ml - 6 U/ml
  • Recovery

    93 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Monkey
  • Product overview

    Abcam’s Soluble TNF Receptor I Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey Soluble TNF Receptor I concentrations in Cell culture supernatant and serum (baboon, cynomolgus).

    Soluble TNF Receptor I specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated Soluble TNF Receptor I detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is then added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the Soluble TNF Receptor I amount of sample captured in plate.

  • Notes

    Standard is recombinant protein produced in E.coli.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119562.

Protocols

References

ab119562 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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