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Abcam’s TNF-alpha Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey TNF-alpha concentrations in cell culture supernatant and serum (baboon, cynomolgus).
TNF-alpha specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a biotinylated TNF-alpha detection. The microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of TNF-alpha captured on the plate.
Standard is recombinant protein produced in E.coli.
|Components||1 x 96 tests|
|20X Assay Buffer Concentrate||1 x 5ml|
|20X Wash Buffer Concentrate||1 x 50ml|
|Adhesive Films||4 units|
|Biotin-Conjugate anti-monkey TNF alpha polyclonal antibody||1 x 70µl|
|Microplate coated with monoclonal antibody to monkey TNF alpha (12 x 8 wells)||1 unit|
|Monkey TNF alpha Standard Lyophilized||2 vials|
|Sample Diluent||1 x 12ml|
|Stop Solution (1M Phosphoric acid)||1 x 15ml|
|Streptavidin-HRP||1 x 150µl|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab119560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119560 has not yet been referenced specifically in any publications.