Anti-mono and dimethyl Arginine antibody [7E6] (ab412)

Overview

  • Product nameAnti-mono and dimethyl Arginine antibody [7E6]
  • Description
    Mouse monoclonal [7E6] to mono and dimethyl Arginine
  • Tested applicationsSuitable for: IP, ChIP, ELISAmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Asymmetrical NG/NG-dimethyl arginine

  • Positive control
    • Methylated histones are an option
  • General notes

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises both dimethyl and monomethyl arginine it can be used in parallel with ab413 (detects dimethyl arginine only) and ab414 (detects monomethyl arginine only) to monitor the exact modification status. A tissue culture supernatant version is available as ab5394.

Properties

Applications

Our Abpromise guarantee covers the use of ab412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ChIP Use 3µl for 106 cells.
ELISA Use a concentration of 0.05 - 0.5 µg/ml.
  • Application notesIs unsuitable for WB.
  • Target

    • Alternative names
      • Di methyl Arginine antibody
      • Dimethyl Arginine antibody
      • Methyl Arginine antibody
      • Mono methyl Arginine antibody
      • Monomethyl Arginine antibody
      see all

    Anti-mono and dimethyl Arginine antibody [7E6] images

    • Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab412 to mono and dimethyl Arginine.

      Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3 µl of ab412 and 4x106 cells were used in each ChIP experiment.

    • RIP140 null adipocytes extracts were suspended in 250 µl of immunoprecipitation buffer (150 mM NaCl,50 mM Tris-HCl (pH 8.0), 1 mM EDTA, 0.2% (v/v) Nonidet P40, 2 mM PMSF, 0.1% (w/v) SDS and a protease-inhibitor cocktail. Protein extracts (200 µg) were incubated with antibodies to RIP140, 14-3-3, or PRMT1 overnight at 4°C, and precipitated with protein G–agarose beads for 1–2 hours. ab412 was then used (amongst other antibodies) in Western Blot. Reduced protein inputs were used for ectopic expression of RIP140 to avoid saturation.

    References for Anti-mono and dimethyl Arginine antibody [7E6] (ab412)

    This product has been referenced in:
    • Yang JH  et al. Arginine methylation of hnRNPK negatively modulates apoptosis upon DNA damage through local regulation of phosphorylation. Nucleic Acids Res 42:9908-24 (2014). Read more (PubMed: 25104022) »
    • Casadio F  et al. H3R42me2a is a histone modification with positive transcriptional effects. Proc Natl Acad Sci U S A 110:14894-9 (2013). Read more (PubMed: 23980157) »

    See all 23 Publications for this product

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Protein A/G
    Sample Human Cell lysate - whole cell (fibroblast)
    Specification fibroblast
    Total protein in input 1000 µg
    Username

    Abcam user community

    Verified customer

    Submitted Jun 19 2014

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    These antibodies are raised against targets that are not species specific. The antibodies would detect the proteins irrespective of species so the Abtrial discount will not be valid.

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