Overview

  • Product nameAnti-mono methyl Arginine antibody [5D1]
    See all mono methyl Arginine primary antibodies
  • Description
    Mouse monoclonal [5D1] to mono methyl Arginine
  • SpecificityThis antibody reacts with free and bound NG-monomethyl arginine. There is no cross reaction with either NG-NG-dimethyl arginine or arginine.
  • Tested applicationsSuitable for: WB, ChIPmore details
  • Species reactivity
    Reacts with: Species independent
  • General notes

    This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.

Properties

Applications

Our Abpromise guarantee covers the use of ab415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ChIP Use 5µl for 106 cells.

Target

  • Alternative names
    • Arginine (mono methyl) antibody

Anti-mono methyl Arginine antibody [5D1] images

  • Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.

    Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 µl of ab415 and 4x106 cells were used in each ChIP experiment.

  • We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het).  A "Yes"  indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result. 

     

    Specific details.

     

    Chromatin source:  Chicken erythrocytes.  Chromatin was formaldehyde crosslinked and then sonicated.  The chromatin was then purified on a CsCl gradient.

     

    ChIP:  The Abcam protocol from the website was employed.  Approximately 4-5 ug of each antibody was used with 30-40 ug of chromatin per ChIP.

     

    PCR:  An Applied Biosystems ABI 7000 Real-time PCR instrument was used.  Taqman probe technology was used.

References for Anti-mono methyl Arginine antibody [5D1] (ab415)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thank you for your phone call. I am glad to hear that for ab109451 the protocol tips did work. I am very sorry that unfortunately, for ab413 and ab415, the tips did not improve your results. However, the details provided enable us to closely monitor th...

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Thank you for your phone call. I am sorry to hear that these 3 antibodies are not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality....

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Thank you for your patience. Ab415, ab414, and ab413 are ascites. It has not been determinded as to whether ab412 and ab413 cross-react with symmetrical Ng/Na - dimethyl arginine.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"