Anti-mono methyl Arginine antibody [5D1] - ChIP Grade (ab415)

Overview

• Product nameAnti-mono methyl Arginine antibody [5D1] - ChIP GradeSee all mono methyl Arginine primary antibodies ...
• Description
  Mouse monoclonal [5D1] to mono methyl Arginine - ChIP Grade
• SpecificityThis antibody reacts with free and bound N^G-monomethyl arginine. There is no cross reaction with either N^G-N^G-dimethyl arginine or arginine.
• Tested applicationsWB, ChIP more details

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
• Concentration information loading...
• PurityProtein A purified
• Primary antibody notes This antibody will be of central importance in analysing the methylation status of chromatin and transcription factors. Since it recognises monomethyl arginine only it can be used in parallel with ab413 (detects dimethyl arginine only) and ab412 (detects both monomethyl and dimethyl arginine) to monitor the exact modification status.
• Clonality Monoclonal
• Clone number5D1
• Myelomaunknown
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Signal Transduction
  • Metabolism
  • Amino Acids

  • Epigenetics and Nuclear Signaling
  • ChIP'ing antibodies
  • ChIP'ing antibodies

  • Epigenetics and Nuclear Signaling
  • Histones
  • H2A
  • Methylated

  • Epigenetics and Nuclear Signaling
  • Histones
  • H4
  • Methylated

  • Epigenetics and Nuclear Signaling
  • Histones
  • H3
  • Methylated

Applications

Target

• Alternative names
  Arginine (mono methyl) antibody

Anti-mono methyl Arginine antibody [5D1] - ChIP Grade images

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  ChIP - Anti-mono methyl Arginine antibody [5D1] - ChIP Grade (ab415)Sylvain Daujat, Tony Kouzarides lab, Cambridge University, UK

  Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure (see protocol) with ab415 to mono methyl Arginine.

  Immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 µl of ab415 and 4x10⁶ cells were used in each ChIP experiment.

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  - mono methyl Arginine antibody [5D1] - ChIP Grade (ab415)

  We looked at chicken chromatin and then analysed 4 active genes (AG1-4), 2 inactive genes (IG1-2) as well as heterochromatin (Het).  A "Yes"  indicates that the antibody immunoprecipitated material well above background and should be regarded as a positive result. 

   

  Specific details.

   

  Chromatin source:  Chicken erythrocytes.  Chromatin was formaldehyde crosslinked and then sonicated.  The chromatin was then purified on a CsCl gradient.

   

  ChIP:  The Abcam protocol from the website was employed.  Approximately 4-5 ug of each antibody was used with 30-40 ug of chromatin per ChIP.

   

  PCR:  An Applied Biosystems ABI 7000 Real-time PCR instrument was used.  Taqman probe technology was used.