Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
Detected in adipose tissue, lung, liver, kidney, brain and heart.
Western blot - Anti-Monoacylglycerol Lipase antibody (ab180016)
All lanes : Anti-Monoacylglycerol Lipase antibody (ab180016) at 1 µg/ml (Milk blocking 3%)
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue Lane 3 : Adipose (Human) Total Protein Lysate - adult normal tissue Lane 4 : Adult Mouse Brown Adipose Tissue Tissue Lysate
Lysates/proteins at 25 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa Observed band size : 33 kDa Additional bands at : 37 kDa (possible non-specific binding),80 kDa (possible non-specific binding).
Exposure time : 16 minutes
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab180016 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.