Anti-Monoacylglycerol Lipase antibody (ab180016)

Overview

  • Product name
    Anti-Monoacylglycerol Lipase antibody
    See all Monoacylglycerol Lipase primary antibodies
  • Description
    Rabbit polyclonal to Monoacylglycerol Lipase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Dog, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Monoacylglycerol Lipase aa 250 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q99685

  • Positive control
    • This antibody gave a positive signal in Mouse brown adipose tissue as well as the following Human tissue lysates: Heart; Liver; Adipose tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab180016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

AP-0017395

Target

  • Function
    Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
  • Tissue specificity
    Detected in adipose tissue, lung, liver, kidney, brain and heart.
  • Pathway
    Glycerolipid metabolism; triacylglycerol degradation.
  • Sequence similarities
    Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 3.1.1.23 antibody
    • HU K5 antibody
    • HU-K5 antibody
    • HUK5 antibody
    • Lysophospholipase homolog antibody
    • Lysophospholipase like antibody
    • Lysophospholipase-like antibody
    • MAGL antibody
    • MGL antibody
    • MGLL antibody
    • MGLL_HUMAN antibody
    • Monoacylglycerol lipase antibody
    • Monoglyceride lipase antibody
    see all

Images

  • All lanes : Anti-Monoacylglycerol Lipase antibody (ab180016) at 1 µg/ml (Milk blocking 3%)

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue
    Lane 3 : Adipose (Human) Total Protein Lysate - adult normal tissue
    Lane 4 : Adult Mouse Brown Adipose Tissue Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 37 kDa (possible non-specific binding),80 kDa (possible non-specific binding).

    Exposure time : 16 minutes

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab180016 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab180016 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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