Anti-Monoacylglycerol Lipase antibody (ab77398)

Overview

  • Product name
    Anti-Monoacylglycerol Lipase antibody
    See all Monoacylglycerol Lipase primary antibodies
  • Description
    Goat polyclonal to Monoacylglycerol Lipase
  • Specificity
    abb77398 is expected to recognize both reported isoforms (NP_009214.1 and NP_001003794.1).
  • Tested applications
    Suitable for: ELISA, IHC-P, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide:

    C-QDLPHLVNADGQY

    , corresponding to internal sequence amino acids 17-29 of Human Monoacylglycerol Lipase (NP_009214.1; NP_001003794.1).

  • Positive control
    • Human Adipose and Brain (Frontal Cortex) lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab77398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-P
WB
IHC-Fr
  • Application notes
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
    • Tissue specificity
      Detected in adipose tissue, lung, liver, kidney, brain and heart.
    • Pathway
      Glycerolipid metabolism; triacylglycerol degradation.
    • Sequence similarities
      Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 3.1.1.23 antibody
      • HU K5 antibody
      • HU-K5 antibody
      • HUK5 antibody
      • Lysophospholipase homolog antibody
      • Lysophospholipase like antibody
      • Lysophospholipase-like antibody
      • MAGL antibody
      • MGL antibody
      • MGLL antibody
      • MGLL_HUMAN antibody
      • Monoacylglycerol lipase antibody
      • Monoglyceride lipase antibody
      see all

    Anti-Monoacylglycerol Lipase antibody images

    • Anti-Monoacylglycerol Lipase antibody (ab77398) at 0.5 µg/ml + Human Adipose lysate (in RIPA buffer) at 35 µg

      Predicted band size : 33 kDa
      Observed band size : 33 kDa
    • Immunohistochemical staining of Monoacylglycerol Lipase using ab77398 on Formaldehyde-fixed paraffin-embedded mouse adipose tissue sections (from around the kidney).  Antigen retrieval step: heat mediated in citric acid HIER. Blocking step: 1% BSA for 10 mins @ rt°C. ab77398 incubated at 1/500 for 2 hours. Secondary Antibody:  biotin labelled anti goat IgG used at 1/200. The submitted image shows white fat deposits around the kidney. Dilution factors vary between 1/300-500. There is a pale positivity in the renal tubular cytoplasm which appears to be specific (at lower dilution factor this is more obvious but, in this block the fat was too strongly immunostained). Section was endogenous biotin-blocked prior to incubation with primary Ab.

      See Abreview

    References for Anti-Monoacylglycerol Lipase antibody (ab77398)

    ab77398 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Tissue sections (Brown adipose tissue)
    Specification
    Brown adipose tissue
    Permeabilization
    Yes - 0.2 % Triton
    Fixative
    Formaldehyde
    Username

    Mrs. Noemi Pasquarelli

    Verified customer

    Submitted Nov 11 2013




    Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab77398 in IHC-Fr zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einset...

    Read More

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unseres Wissens nach wurde ab77398 Anti-Monoacylglycerol Lipase antibody bisher noch nicht in IHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney adipose tissue)
    Specification
    kidney adipose tissue
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid HIER
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 21 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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