Recombinant
RabMAb

Anti-Monoamine Oxidase A antibody [EPR7101] (ab126751)

Overview

  • Product name
    Anti-Monoamine Oxidase A antibody [EPR7101]
    See all Monoamine Oxidase A primary antibodies
  • Description
    Rabbit monoclonal [EPR7101] to Monoamine Oxidase A
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HepG2, NCI-H460 and fetal liver lysates; Human colon and kidney tissues
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
    • Tissue specificity
      Heart, liver, duodenum, blood vessels and kidney.
    • Involvement in disease
      Defects in MAOA are the cause of Brunner syndrome (BRUNS) [MIM:300615]. Brunner syndrome is a form of X-linked non-dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior.
    • Sequence similarities
      Belongs to the flavin monoamine oxidase family.
    • Cellular localization
      Mitochondrion outer membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Amine oxidase [flavin containing] A antibody
      • Amine oxidase [flavin-containing] A antibody
      • AOFA antibody
      • AOFA_HUMAN antibody
      • EC 1.4.3.4 antibody
      • MAO A antibody
      • MAO-A antibody
      • maoA antibody
      • Monoamine oxidase A antibody
      • Monoamine oxidase type A antibody
      see all

    Images

    • ab126751 staining Monoamine Oxidase A in the human cell line HepG2 (human hepatocellular carcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Anti-Monoamine Oxidase A antibody [EPR7101] (ab126751) at 1/2000 dilution + Mouse brain cortex tissue lysate - whole at 20 µg

      Secondary
      HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 60 kDa
      Observed band size : 60 kDa


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      See Abreview

    • All lanes : Anti-Monoamine Oxidase A antibody [EPR7101] (ab126751) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : NCI-H460 lysate
      Lane 3 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 60 kDa
    • ab126751, at 1/50 dilution, staining Monoamine Oxidase A in paraffin embedded Human colon tissue by Immunohistochemistry.
    • ab126751, at 1/50 dilution, staining Monoamine Oxidase A in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Stefanovic B  et al. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. Eur Neuropsychopharmacol 26:1629-37 (2016). WB ; Rat . Read more (PubMed: 27499503) »
    • Popova D  et al. Non-Serotonergic Neurotoxicity by MDMA (Ecstasy) in Neurons Derived from Mouse P19 Embryonal Carcinoma Cells. PLoS One 11:e0166750 (2016). WB ; Mouse . Read more (PubMed: 27861613) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Mouse Tissue lysate - whole (Brain cortex)
    Specification
    Brain cortex
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 24 2013

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain, skin, small and large intestines)
    Specification
    Brain, skin, small and large intestines
    Permeabilization
    No
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Jul 05 2013

    Thank you for contacting us. The antibody has not been tested in frozen sections but I would expect it to work. Typically antibodies tested in paraffin sections do work in frozen sections, although the reverse is not necessarily true. We do put on the ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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