Anti-Monoamine Oxidase B antibody (ab118865)

Overview

  • Product nameAnti-Monoamine Oxidase B antibody
    See all Monoamine Oxidase B primary antibodies
  • Description
    Goat polyclonal to Monoamine Oxidase B
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Pig
  • Immunogen

    ab118865 was raised against a synthetic peptide corresponding to a region within internal sequence amino acids 347-359 (

    C-HKARKLARLTKEE

    ) of Human Monoamine Oxidase B.

  • Positive control
    • Human brain cortex tissue, Human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab118865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 59 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-Monoamine Oxidase B antibody images

  • ab118865 at 5 µg/ml staining Monoamine Oxidase B in Paraffin-embedded Human brain cortex tissue by immunohistochemistry. After incubation with ab118865 slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
  • All lanes : Anti-Monoamine Oxidase B antibody (ab118865) at 1 µg/ml

    Lane 1 : Human brain lysate without blocking peptide
    Lane 2 : Human brain lysate with blocking peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 59 kDa

References for Anti-Monoamine Oxidase B antibody (ab118865)

ab118865 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (BRAIN)
Permeabilization Yes - 0.1 % TRITION X
Specification BRAIN
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Pl Abhilash

Verified customer

Submitted Jun 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (mouse brain(cortex))
Specification mouse brain(cortex)
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.2% Triton-x 100
Username

Abcam user community

Verified customer

Submitted Feb 29 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"