Overview

  • Product name
    Anti-Monoamine Oxidase B antibody
    See all Monoamine Oxidase B primary antibodies
  • Description
    Goat polyclonal to Monoamine Oxidase B
  • Specificity
    This antibody is not expected to cross-react with Monoamine oxidase A.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-HKARKLARLTKEE

    , corresponding to internal sequence amino acids 347-359 of Human Monoamine Oxidase B

  • Positive control
    • Human Brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab47988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 21873469

Target

Images

  • All lanes : Anti-Monoamine Oxidase B antibody (ab47988) at 1 µg/ml

    Lane 1 : Human Brain lysate (35µg protein in RIPA buffer) with no blocking with the immunizing peptide
    Lane 2 : Human Brain lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide


    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-Monoamine Oxidase B antibody (ab47988) at 0.5 µg/ml + Human brain (hippocampus) lysate in RIPA buffer. at 35 µg
    Developed using the ECL technique

    Predicted band size : 59 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Francis H  et al. Inhibition of histidine decarboxylase ablates the autocrine tumorigenic effects of histamine in human cholangiocarcinoma. Gut 61:753-64 (2012). ICC/IF ; Human . Read more (PubMed: 21873469) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your inquiry. Please be aware that our ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide. The protocol is as follows: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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