Overview

  • Product nameAnti-Monoamine Oxidase B antibody
    See all Monoamine Oxidase B primary antibodies
  • Description
    Goat polyclonal to Monoamine Oxidase B
  • SpecificityThis antibody is not expected to cross-react with Monoamine oxidase A.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-HKARKLARLTKEE

    , corresponding to internal sequence amino acids 347-359 of Human Monoamine Oxidase B

  • Positive control
    • Human Brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab47988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 21873469

Target

Anti-Monoamine Oxidase B antibody images

  • All lanes : Anti-Monoamine Oxidase B antibody (ab47988) at 1 µg/ml

    Lane 1 : Human Brain lysate (35µg protein in RIPA buffer) with no blocking with the immunizing peptide
    Lane 2 : Human Brain lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide


    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-Monoamine Oxidase B antibody (ab47988) at 0.5 µg/ml + Human brain (hippocampus) lysate in RIPA buffer. at 35 µg
    Developed using the ECL technique

    Predicted band size : 59 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References for Anti-Monoamine Oxidase B antibody (ab47988)

This product has been referenced in:
  • Francis H  et al. Inhibition of histidine decarboxylase ablates the autocrine tumorigenic effects of histamine in human cholangiocarcinoma. Gut 61:753-64 (2012). ICC/IF ; Human . Read more (PubMed: 21873469) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry. Please be aware that our ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide. The protocol is as follows: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"