Overview

  • Product nameAnti-Monoamine Oxidase B antibody
    See all Monoamine Oxidase B primary antibodies
  • Description
    Mouse polyclonal to Monoamine Oxidase B
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Marmoset (common)
  • Immunogen

    Full length human protein (NP_000889.3)

  • Positive control
    • Human liver lysate. Human kidney tissue. heLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
    • Sequence similaritiesBelongs to the flavin monoamine oxidase family.
    • Cellular localizationMitochondrion outer membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adrenalin oxidase antibody
      • Amine oxidase (flavin containing) antibody
      • Amine oxidase [flavin-containing] B antibody
      • AOFB_HUMAN antibody
      • MAO, brain antibody
      • MAO, platelet antibody
      • MAO-B antibody
      • MAOB antibody
      • MGC26382 antibody
      • Monoamine oxidase B antibody
      • Monoamine oxidase type B antibody
      • OTTHUMP00000023166 antibody
      • Tyramine oxidase antibody
      see all

    Anti-Monoamine Oxidase B antibody images

    • Anti-Monoamine Oxidase B antibody (ab88510) at 1 µg/ml + Human liver lysate at 50 µg

      Predicted band size : 59 kDa
      Observed band size : 59 kDa
    • All lanes : Anti-Monoamine Oxidase B antibody (ab88510) at 1 µg/ml

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 59 kDa
      Observed band size : 59 kDa
    • Purified ab88510 at 3ug/ml staining Monoamine Oxidase B in formalin-fixed, paraffin embedded Human kidney tissue.
    • Immunofluorescent staining of Monoamine Oxidase B in HeLa cells using purified ab88510 at 10ug/ml.

    References for Anti-Monoamine Oxidase B antibody (ab88510)

    This product has been referenced in:
    • Grouzmann E  et al. Monoamine oxidase a down-regulation contributes to high metanephrine concentration in pheochromocytoma. J Clin Endocrinol Metab 97:2773-81 (2012). Human . Read more (PubMed: 22569243) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
    Sample Marmoset (common) Tissue lysate - whole (brain)
    Specification brain
    Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 05 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"