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ab109912 (MS747) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate MAOB from all other peroxidases and oxidases (including MAOA) in a tissue or cultured cell sample. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated MAOB is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
Store Fluorophore and benzylamine at -80°C. Store all other components store at 4°C.
|Components||1 x 96 tests|
|100X Benzylamine Substrate||1 x 0.25ml|
|100X Detector Antibody||1 x 0.125ml|
|100X HRP Label||1 x 0.125ml|
|10X Blocking Solution||1 x 10ml|
|12-channel reagent reservoirs||1 unit|
|20X Wash Buffer||1 x 25ml|
|500X Fluorophore||1 x 50µl|
|500X Peroxidase||1 x 50µl|
|96-well Microplate (12 strips)||1 unit|
|Extraction Buffer||1 x 15ml|
|HRP Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab109912 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent dilution.|
ab109912 has not yet been referenced specifically in any publications.