Anti-MCT1/Monocarboxylic acid transporter 1 antibody (ab90582)
Key features and details
- Mouse polyclonal to MCT1/Monocarboxylic acid transporter 1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MCT1/Monocarboxylic acid transporter 1 antibody
See all MCT1/Monocarboxylic acid transporter 1 primary antibodies -
Description
Mouse polyclonal to MCT1/Monocarboxylic acid transporter 1 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (2) |
Use a concentration of 10 µg/ml.
|
WB | (3) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
|
Notes |
---|
ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. |
Target
-
Function
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. -
Tissue specificity
Widely expressed in normal and in cancer cells. -
Involvement in disease
Symptomatic deficiency in lactate transport
Familial hyperinsulinemic hypoglycemia 7 -
Sequence similarities
Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6566 Human
- Omim: 600682 Human
- SwissProt: P53985 Human
- Unigene: 75231 Human
-
Alternative names
- FLJ36745 antibody
- HHF7 antibody
- MCT 1 antibody
see all
Images
-
All lanes : Anti-MCT1/Monocarboxylic acid transporter 1 antibody (ab90582) at 1 µg/ml
Lane 1 : Monocarboxylic acid transporter 1- transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 54 kDa -
ab90582 at 10µg/ml staining Monocarboxylic acid transporter 1 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (22)
ab90582 has been referenced in 22 publications.
- Ippolito L et al. Lactate Rewires Lipid Metabolism and Sustains a Metabolic-Epigenetic Axis in Prostate Cancer. Cancer Res 82:1267-1282 (2022). PubMed: 35135811
- Sun Y et al. Prognostic implications of combined high expression of CD47 and MCT1 in breast cancer: a retrospective study during a 10-year period. Transl Cancer Res 11:29-42 (2022). PubMed: 35261882
- Peltanová B et al. mRNA Subtype of Cancer-Associated Fibroblasts Significantly Affects Key Characteristics of Head and Neck Cancer Cells. Cancers (Basel) 14:N/A (2022). PubMed: 35565415
- Longhitano L et al. Lactate Induces the Expressions of MCT1 and HCAR1 to Promote Tumor Growth and Progression in Glioblastoma. Front Oncol 12:871798 (2022). PubMed: 35574309
- Li L et al. Multi-omics profiling of collagen-induced arthritis mouse model reveals early metabolic dysregulation via SIRT1 axis. Sci Rep 12:11830 (2022). PubMed: 35821263