Anti-Monocarboxylic acid transporter 1 antibody (ab93048)

Overview

  • Product name
    Anti-Monocarboxylic acid transporter 1 antibody
    See all Monocarboxylic acid transporter 1 primary antibodies
  • Description
    Rabbit polyclonal to Monocarboxylic acid transporter 1
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide corresponding to N terminal residues of Mouse Monocarboxylic acid transporter 1

Properties

Applications

Our Abpromise guarantee covers the use of ab93048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
  • Tissue specificity
    Widely expressed in normal and in cancer cells.
  • Involvement in disease
    Symptomatic deficiency in lactate transport
    Familial hyperinsulinemic hypoglycemia 7
  • Sequence similarities
    Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ36745 antibody
    • HHF7 antibody
    • MCT 1 antibody
    • MCT antibody
    • MGC44475 antibody
    • Monocarboxylate transporter 1 antibody
    • Monocarboxylate transporter antibody
    • Monocarboxylate transporter isoform 1 antibody
    • Monocarboxylic acid transporter 1 antibody
    • MOT1_HUMAN antibody
    • Slc16a1 antibody
    • SLC16A1 protein antibody
    • Solute carrier family 16 (monocarboxylic acid transporters) member 1 antibody
    • Solute carrier family 16 member 1 (monocarboxylic acid transporter 1) antibody
    • Solute carrier family 16 member 1 antibody
    see all

References for Anti-Monocarboxylic acid transporter 1 antibody (ab93048)

ab93048 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Chicken Tissue lysate - whole (Breast)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Breast
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 23°C
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Verified customer

Submitted Jun 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
Other
Permeabilization
Yes - tween 20
Specification
brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Oct 05 2015

We unfortunately do not commercially offer blocking peptide for this antibody so I am sorry we may not be able ot fulfil your request this time.

We can however offer blocking peptide for ab179832, please do let me know if you are interested.

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Sample
Rat Cell (astrocytes)
Specification
astrocytes
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 27 2013


The lab let me know that this antibody has been tested in mouse over-expressed cell line by WB/ELISA, it thus should work with endogenous mouse samples.

I have added mouse as tested species and thus we would guarantee it.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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