Anti-Monocyte + Granulocyte antibody [74-22-15] (FITC) (ab24885)

Overview

  • Product name
    Anti-Monocyte + Granulocyte antibody [74-22-15] (FITC)
  • Description
    Mouse monoclonal [74-22-15] to Monocyte + Granulocyte (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody reacts with >90% of pig monocytes and granulocytes and <5% of peripheral blood lymphocytes.
  • Tested applications
    Suitable for: Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    dd miniature swine thymocytes

  • General notes


    This clone was clustered as anti-SWC3a at the First International Swine CD Workshop.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by July 2018 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    This clone was clustered as anti-SWC3a at the First International Swine CD Workshop.
  • Clonality
    Monoclonal
  • Clone number
    74-22-15
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    A monocyte is a leukocyte, part of the human body's immune system that protects against blood-borne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues. Monocytes are usually identified in stained smears by their large bi-lobed nucleus. Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

Anti-Monocyte + Granulocyte antibody [74-22-15] (FITC) images

  • Fresh heparinized blood from a healthy adult pig was stained with ab24885 at 1 µg/106 cells. Red blood cells were lyzed with ammonium chloride and the cells were then fixed in 1% paraformaldehyde. Granulocytes were gated and analyzed on a FACScanTM flow cytometer (BDIS, San Jose, CA).

References for Anti-Monocyte + Granulocyte antibody [74-22-15] (FITC) (ab24885)

This product has been referenced in:
  • Chen X  et al. Retinoic acid facilitates inactivated transmissible gastroenteritis virus induction of CD8(+) T-cell migration to the porcine gut. Sci Rep 6:24152 (2016). Flow Cyt . Read more (PubMed: 27080036) »
  • Fusconi M  et al. Is montgomery tracheal safe-T-tube clinical failure induced by biofilm? Otolaryngol Head Neck Surg 149:269-76 (2013). Human . Read more (PubMed: 23649498) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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