Primary antibody notesThis antibody reacts with >90% of pig monocytes and granulocytes and <5% of peripheral blood lymphocytes. This clone was clustered as anti-SWC3a at the First International Swine CD Workshop.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells.
Use at an assay dependent dilution.
RelevanceA monocyte is a leukocyte, part of the human body's immune system that protects against blood-borne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues. Monocytes are usually identified in stained smears by their large bi-lobed nucleus.
Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
References for Anti-Monocyte + Granulocyte antibody [74-22-15] (Phycoerythrin) (ab25684)
has not yet been referenced specifically in any publications.
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