Mouse lymph node stroma
Our Abpromise guarantee covers the use of ab33451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 24449804|
|Flow Cyt||Use at an assay dependent concentration.
Membrane permeabilisation is required for this application.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ab33451 staining Monocyte + Macrophage in mouse aorta tissue by Immunohistochemistry (Frozen sections). The sections were fixed in acetone prior to blocking with 5% serum for 30 minutes at room temperature. The primary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour. A FITC-conjugated rabbit anti-rat polyclonal was used as the secondary antibody, diluted 1/400.
Endothelium is stained with Von Willebrand Factor (ab6994) and counterstained with DAPI.
ab33451 staining mouse brain tissue sections by IHC-FoFr. Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 18 hours at 4°C. A HRP conjugated goat anti-rat antibody, diluted 1/500, was used as the secondary.
This antibody labels perivascular (left) and subpial (right) macs in normal brain tissue.