Anti-Monocyte + Macrophage antibody [MOMA-2] (ab33451)

Overview

  • Product nameAnti-Monocyte + Macrophage antibody [MOMA-2]
    See all Monocyte + Macrophage primary antibodies
  • Description
    Rat monoclonal [MOMA-2] to Monocyte + Macrophage
  • SpecificityThis antibody recognises an intracellular antigen of mouse macrophages and monocytes. It reacts strongly with macrophages in lymphoid organs in all mouse strains tested. Staining may also be seen on the epithelium of mucosa.
  • Tested applicationsSuitable for: IHC-P, IHC-FoFr, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse lymph node stroma

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberMOMA-2
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 24449804
IHC-FoFr 1/50.
Flow Cyt Use at an assay dependent concentration.

Membrane permeabilisation is required for this application.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/25.

Target

  • RelevanceMonocyte and macrophage are white blood cells that roam the body tissues engulfing foreign organisms. A monocyte is a leukocyte, part of the human body's immune system that protects against blood-borne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues. Monocytes are usually identified in stained smears by their large bi-lobed nucleus. Macrophages are cells within the tissues that originate from specific white blood cells called monocytes. Monocytes and macrophages are phagocytes, acting in both nonspecific defense (or innate immunity) as well as specific defense (or cell-mediated immunity) of vertebrate animals. Their role is to phagocytize (engulf and then digest) cellular debris and pathogens either as stationary or mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen.
  • Alternative names
    • Macrophages antibody
    • Monocytes antibody

Anti-Monocyte + Macrophage antibody [MOMA-2] images

  • ab33451 staining mouse brain tissue sections by IHC-FoFr.  Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C.  The primary antibody was diluted 1/50 and incubated with the sample for 18 hours at 4°C.  A HRP conjugated goat anti-rat antibody, diluted 1/500, was used as the secondary. 

    This antibody labels perivascular (left) and subpial (right) macs in normal brain tissue.

    See Abreview

  • ab33451 staining Monocyte + Macrophage in mouse suprarenal aorta sections by Immunohistonchemistry (Frozen sections).
    Serial cryostat sections 7 µm thick were cut from suprarenal aortas with aneurysms prior to staining for inflammatory cells. Serial frozen sections were air-dried, fixed in acetone for 10 minutes at -20°C, air dried and rehydrated with PBS before being incubated in 3% H2O2/0.1% sodium azide/PBS to block endogenous peroxidase. For macrophage detection, sections were blocked in 2% normal goat serum in PBS followed by staining using ab33451. Slides were incubated in the peroxidase substrate 3, 3'-diamminobenzidine, counterstained in Mayer's Haematoxylin, dehydrated, cleared in xylene and mounted in Depex mounting medium.
  • ab33451 staining Monocyte + Macrophage in Mouse aorta tissue by Immunohistochemistry (Frozen sections). The sections were fixed in acetone prior to blocking with 5% serum for 30 minutes at room temperature. The primary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour. A FITC-conjugated Rabbit anti-Rat polyclonal was used as the secondary antibody, diluted 1/400.
    Endothelium is stained with Von Willebrand Factor (ab6994) and counterstained with DAPI

    See Abreview

References for Anti-Monocyte + Macrophage antibody [MOMA-2] (ab33451)

This product has been referenced in:
  • Lim H  et al. Anti-atherogenic effect of Humulus japonicus in apolipoprotein E-deficient mice. Int J Mol Med 38:1101-10 (2016). Read more (PubMed: 27600281) »
  • Billon C  et al. Inhibition of RORa/? suppresses atherosclerosis via inhibition of both cholesterol absorption and inflammation. Mol Metab 5:997-1005 (2016). Read more (PubMed: 27689012) »

See all 16 Publications for this product

Product Wall

Thank you for contacting us.

ab7815 and ab33451 have been tested with Frozen sections only and we do not have any empirical data to support their performance with paraffin tissues.

I have found the following ICAM1 and Monocyte + mac...

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The following reference appears to be the source of the statement on the ab33451 datasheet (anti-macrophage/monocyte, MOMA-2 clone) regarding reactivity with mucosa epithelium:

The localization of macr...

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I am very pleased to hear you would like to accept our offer and test abab33451 in IHC-P. This code will give you 1 free PRIMARY ANTIBODY before the ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Dec 08 2011

Thanks so much for keeping me updated and letting me know how the replacement antibody worked. I am so happy to hear that the replacement antibody ab53444 worked in your staining! Perhaps the non-specific staining could be reduced by increasing the blo...

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Thanks for your call today and for letting us know about the trouble with ab33451. I am sorry to hear that this antibody did not produce the expected results. As we discussed, I am sending a free of charge vial of ab53444 on the order ***, whic...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Aorta)
Specification Aorta
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (normal brain)
Specification normal brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step TNB as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"