Overview

  • Product name
  • Description
    Mouse polyclonal to MORC3
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human protein

  • Positive control
    • Hela cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 107 kDa.

Target

  • Function
    Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism (PubMed:20501696). Sumoylated MORC3-NBs can also associate with PML-NBs (PubMed:20501696). Recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity (PubMed:17332504). Binds RNA in vitro (PubMed:11927593). May be required for influenza A transcription during viral infection (PubMed:26202233).
  • Tissue specificity
    Expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney.
  • Sequence similarities
    Contains 1 CW-type zinc finger.
  • Post-translational
    modifications
    Sumoylation is involved in interaction with PML and localization to PML nuclear bodies.
  • Cellular localization
    Nucleus, nucleoplasm. Nucleus matrix. Nucleus, PML body. Also found in PML-independent nuclear bodies. Localization to nuclear bodies is ATP-dependent.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0136 antibody
    • Microrchidia 3 antibody
    • MORC family CW type zinc finger 3 antibody
    • MORC family CW type zinc finger protein 3 antibody
    • MORC family CW-type zinc finger protein 3 antibody
    • MORC3 antibody
    • MORC3_HUMAN antibody
    • Nuclear matrix protein 2 antibody
    • Nuclear matrix protein NXP2 antibody
    • NXP2 antibody
    • ZCW5 antibody
    • ZCWCC3 antibody
    • Zinc finger CW type coiled coil domain 3 antibody
    • Zinc finger CW type coiled coil domain protein 3 antibody
    • Zinc finger CW type with coiled coil domain 3 antibody
    • Zinc finger CW-type coiled-coil domain protein 3 antibody
    see all

Images

  • Anti-MORC3 antibody (ab76813) at 1/500 dilution + nuclear extracts from Hela cells at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 107 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-MORC3 antibody (ab76813) at 1/500 dilution

    Lane 1 : MORC3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 107 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Ver LS  et al. The Cellular Factor NXP2/MORC3 Is a Positive Regulator of Influenza Virus Multiplication. J Virol 89:10023-30 (2015). Read more (PubMed: 26202233) »
  • Ichimura Y  et al. Anti-NXP2 autoantibodies in adult patients with idiopathic inflammatory myopathies: possible association with malignancy. Ann Rheum Dis : (2012). WB ; Human . Read more (PubMed: 22258483) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab76813.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up