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Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse AKT2.
Both capture and detector antibodies are rabbit monoclonal antibodies.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab208986) which uses the same antibody pair.
To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.
The capture antibody is glycerol free. The detector antibody contains glycerol.
|Components||10 x 96 tests||2 x 96 tests|
|Mouse AKT2 Capture Antibody||1 x 100µg||1 x 20µg|
|Mouse AKT2 Detector Antibody||1 x 25µg||1 x 5µg|
|Mouse AKT2 Lyophilized Protein||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab216054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab216054 has not yet been referenced specifically in any publications.