Overview

  • Product name
    Mouse Albumin ELISA Kit
    See all Albumin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type
    Serum, Plasma, Tissue Lysate
  • Assay type
    Competitive
  • Sensitivity
    = 0.09 µg/ml
  • Range
    0.098 µg/ml - 25 µg/ml
  • Recovery

    98 %

  • Assay time
    2h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Product overview

    Abcam’s Albumin Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse Albumin levels in plasma and serum.

    An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with PBS or TBS buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Albumin measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD).
  • Albumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve Using ab108791.

Protocols

References

ab108791 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

This ELISA kit was originally optimized for serum and plasma but our lab recently validated it for use with lysates. This is why there is not a lysis buffer included with the kit but you can use RIPA buffer.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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