Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)

Overview

  • Product nameMouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit
    See all HRP/DAB detection kit kits
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

  • Notes

    DAB is a suspected carcinogen. Handle with care.

    Contains hydrogen peroxide.

  • Tested applicationsSuitable for: IHC-Pmore details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 15 ml
    50x DAB Chromogen 1 x 0.5ml
    Biotinylated Goat Anti-Polyvalent 1 x 15ml
    DAB substrate 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research areas
  • RelevanceHRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

Our Abpromise guarantee covers the use of ab64264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit images

  • Staining of human estrogen receptor in breast carcinoma tissue using ab16660 - Rabbit monoclonal [SP1] to Estrogen Receptor, and ab64246 - Mouse & Rabbit HRP/DAB Detection Kit.

References for Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)

This product has been referenced in:
  • Alonso-Orgaz S  et al. Proteomic characterization of human coronary thrombus in patients with ST-segment elevation acute myocardial infarction. J Proteomics 109C:368-381 (2014). Read more (PubMed: 25065646) »
  • Ding Z  et al. LOX-1 - dependent mitochondrial DNA damage and NLRP3 activation during systemic inflammation in mice. Biochem Biophys Res Commun 451:637-43 (2014). Mouse . Read more (PubMed: 25130466) »

See all 6 Publications for this product

Product Wall

IHC-P Mouse pancreas

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Diseased mouse pancreas tissue labeled with REST / NRSF (ab202962) at 1/100 dilution - 1 hr RT, followed by conjugation to the secondary antibody and DAB staining
Username

Abcam user community

Verified customer

Submitted Aug 04 2016

Anti-CCR2 antibody

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Detection of anti-CCR2 (monoclonal anti-rabbit) in human paraffin sections using HRP/DAB detection kit.
Username

Abcam user community

Verified customer

Submitted Feb 10 2016


The pretreatment note in the protocol of the IHC kit refers to the antigen retrieval step that is required for most antibody to achieve a successful IHC-P staining.
For both of these antibodies (ab14440 and ab52894) I can recommend to use Sod...

Read More

Thank you for contacting us. This kit can be used for frozen samples as well. Please skip the first step in the protocol.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

This is confusing. ab113316 is a kit not antibody, customer would not be requiring a DAB kit for this.

For ab101854, ab80436 will be a good product.

Thanks!

Thank you for contacting us.

The kits ab80436 and ab64264, both will be OK to use with ab10148 only because it is rabbit antibody, you can select any one of them. Please note that these kits cannot be used with ab75714 because it is a chicken ...

Read More


Yes, Mouse and Rabbit Specific HRP/DAB (ABC) detection IHC kit (ab64264) and AEC Substrate System (Ready to Use) (ab64252) can be used with rat sections.

Thank you for your response.

The protocol seems to be fine and the antibody should work at a dilution of 1/100. At this stage it is difficult to identify the source of the problem, it could be the product has been damaged during storage or sh...

Read More

Thank you for your response and for confirmation.

It was important to know if the detection system was working fine (ab64264). This means that the problem comes from the primary antibody (ab2428).

I can offer you and your troubled c...

Read More

Thank you for your response.

As I understand ab64264: Mouse and Rabbit Specific HRP/DAB (ABC) detection IHC kit which was used as detection worked fine.

The customer is having problem with only the primary antibody ab2428.

Read More

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"