Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit (ab64264)

Overview

  • Product name
    Mouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit
    See all HRP/DAB detection kit kits
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

  • Notes

    DAB is a suspected carcinogen. Handle with care.

    Contains hydrogen peroxide.

  • Tested applications
    Suitable for: IHC-Pmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 15 ml
    50x DAB Chromogen 1 x 0.5ml
    Biotinylated Goat Anti-Polyvalent 1 x 15ml
    DAB substrate 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research areas
  • Relevance
    HRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.

Applications

Our Abpromise guarantee covers the use of ab64264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Images

  • Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).

  • Staining of human estrogen receptor in breast carcinoma tissue using ab16660 - Rabbit monoclonal [SP1] to Estrogen Receptor, and ab64246 - Mouse & Rabbit HRP/DAB Detection Kit.

References

This product has been referenced in:
  • Li B  et al. Beneficial Effects of Paeoniflorin Enriched Extract on Blood Pressure Variability and Target Organ Damage in Spontaneously Hypertensive Rats. Evid Based Complement Alternat Med 2017:5816960 (2017). Read more (PubMed: 28243310) »
  • Pang M  et al. Gypenosides Inhibits Xanthine Oxidoreductase and Ameliorates Urate Excretion in Hyperuricemic Rats Induced by High Cholesterol and High Fat Food (Lipid Emulsion). Med Sci Monit 23:1129-1140 (2017). Read more (PubMed: 28258276) »

See all 14 Publications for this product

Customer reviews and Q&As

IHC-P Mouse pancreas

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Diseased mouse pancreas tissue labeled with REST / NRSF (ab202962) at 1/100 dilution - 1 hr RT, followed by conjugation to the secondary antibody and DAB staining
Username

Abcam user community

Verified customer

Submitted Aug 04 2016

Anti-CCR2 antibody

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Detection of anti-CCR2 (monoclonal anti-rabbit) in human paraffin sections using HRP/DAB detection kit.
Username

Abcam user community

Verified customer

Submitted Feb 10 2016


The pretreatment note in the protocol of the IHC kit refers to the antigen retrieval step that is required for most antibody to achieve a successful IHC-P staining.
For both of these antibodies (ab14440 and ab52894) I can recommend to use Sod...

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This is confusing. ab113316 is a kit not antibody, customer would not be requiring a DAB kit for this.

For ab101854, ab80436 will be a good product.

Thanks!

Thank you for contacting us.

The kits ab80436 and ab64264, both will be OK to use with ab10148 only because it is rabbit antibody, you can select any one of them. Please note that these kits cannot be used with ab75714 because it is a chicken ...

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Yes, Mouse and Rabbit Specific HRP/DAB (ABC) detection IHC kit (ab64264) and AEC Substrate System (Ready to Use) (ab64252) can be used with rat sections.

Thank you for your enquiry.

The list of components of this kit can be found on the online datasheet:
http://www.abcam.com/index.html?datasheet=64264 (or use the following: http://www.abcam.com/index.html?datasheet=64264).

50x...

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Thank you for contacting us.

The protocol mentioned described on the datasheet refers to an Abreview from a previous user of this antibody. Different protocols may also work.

If you are using ab64264 kit, there is no need to use an ...

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Thank you for your enquiry which has been forwarded to the Scientific support team.

I am sorry, it is regrettably not possible toprovide this particular component separately.

If you have any further questions, please do not hesita...

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Thank you for your inquiry.

Since the working dilutions of antibodies are not a fixed value but have to optimised by the endusers themselves according to experimental condition, I cannot give you absolute numbers. Even when the pre-diluted an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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