Overview

  • Product nameMouse BMP2 ELISA Kit
    See all BMP2 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Other biological fluids, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    62.5 pg/ml - 4000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s mouse BMP2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of mouse BMP2 in bone tissue, cell culture supernatants and serum.

    A BMP2 specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for BMP2 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse BMP2 amount of sample captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionInduces cartilage and bone formation.
  • Tissue specificityParticularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
  • Sequence similaritiesBelongs to the TGF-beta family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • BDA2
    • BMP-2
    • BMP-2A
    • Bmp2
    • BMP2_HUMAN
    • BMP2A
    • Bone morphogenetic protein 2
    • Bone morphogenetic protein 2A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse BMP2 ELISA Kit images

  • Representative Standard Curve using ab119582.

Protocols

References for Mouse BMP2 ELISA Kit (ab119582)

This product has been referenced in:
  • Mai Z  et al. Single bout short duration fluid shear stress induces osteogenic differentiation of MC3T3-E1 cells via integrin ß1 and BMP2 signaling cross-talk. PLoS One 8:e61600 (2013). Read more (PubMed: 23593489) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"