Mouse brain tissue lysate - total protein (0 days) (ab7188)

Overview

  • Product nameMouse brain tissue lysate - total protein (0 days)
    See all Brain lysates
  • Description
    Mouse brain total protein lysate
  • General notes

    Source: Outbred NIH-Swiss Albino mice

    The mouse brain tissue was collected from zero (0) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applicationsSuitable for: WBmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab7188 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Ready to load on SDS-PAGE for Western blotting, 10-20 µg per lane is recommended for mini gel.

    References for Mouse brain tissue lysate - total protein (0 days) (ab7188)

    This product has been referenced in:
    • Jennings JS  et al. Circulating ghrelin and leptin concentrations and growth hormone secretagogue receptor abundance in liver, muscle, and adipose tissue of beef cattle exhibiting differences in composition of gain. J Anim Sci 89:3954-72 (2011). WB . Read more (PubMed: 21724941) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. Yes, ab7942 (rat brain lysate) or ab7188 (mouse brain lysate) could be used as a positive control for ab3908 and ab5683. The previous email that I sent about ab10772 [CaMKII alpha peptide - phospho T286 (phospho and non-...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"