Mouse brain tissue lysate - total protein (ab30151)

Overview

  • Product nameMouse brain tissue lysate - total protein
    See all Brain lysates
  • Description
    Mouse brain total protein lysate
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab30151 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Mouse brain tissue lysate - total protein (ab30151)

    ab30151 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. These two products are supplied as adult lysates, all older than 6 weeks , so they should be suitable for your studies.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need an...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for your reply.


    I have a few additional suggestions. I am not sure if my colleague had mentioned those to you:

    1) You can check the transfer with Ponceau S (which is a reversible membrane stain) to be sure that you tr...

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    Thank you for contacting us.
    I am sorry to hear that the antibody might not be working as expected.

    As we discussed over the phone, I would recommend the following positive control:

    ab30151 (http://www.abcam.com/brain-mouse-who...

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    Thank you for contacting us. I presume you are looking for positive control used in western blot. Our customers have used this ab successfully with mouse brain tissue and 293 cells lysates. Please check the Abreviews. The Mouse brain lysates ca...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"