The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-BRN3A antibody (ab30880)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Brain specific homeobox/POU domain protein 3A
Brain-specific homeobox/POU domain protein 3A
Homeobox/POU domain protein RDC 1
Homeobox/POU domain protein RDC-1
Homeobox/POU domain protein RDC1
POU class 4 homeobox 1
POU Domain Class 4 Transcription Factor 1
transcription factor 1
FunctionProbable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Tissue specificityBrain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
Sequence similaritiesBelongs to the POU transcription factor family. Class-4 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
Developmental stageExpression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.