The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Calcium/calmodulin dependent protein kinase (CaM kinase) II beta
Calcium/calmodulin dependent protein kinase II beta
Calcium/calmodulin dependent protein kinase IIB
Calcium/calmodulin dependent protein kinase type II beta chain
calcium/calmodulin-dependent protein kinase II beta
Calcium/calmodulin-dependent protein kinase type II subunit beta
CaM kinase II beta chain
CaM kinase II beta subunit
CaM kinase II subunit beta
CaM-kinase II beta chain
CaMK II beta subunit
CaMK II subunit beta
CaMK-II subunit beta
CaMKII beta subunit
Proline rich calmodulin dependent protein kinase
proline rich calmodulin-dependent protein kinase
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.