Mouse Cathepsin B ELISA Kit (ab119585)

Overview

  • Product nameMouse Cathepsin B ELISA Kit
    See all Cathepsin B kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/well
  • Range
    156 pg/well - 10000 pg/well
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Mouse Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Mouse Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin B specific rat monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the mouse Cathepsin B amount of sample captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similaritiesBelongs to the peptidase C1 family.
  • Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Alternative names
    • Amyloid precursor protein secretase
    • APP secretase
    • APPS
    • CATB_HUMAN
    • Cathepsin B heavy chain
    • Cathepsin B1
    • CathepsinB
    • CPSB
    • CTSB
    • cysteine protease
    • OTTHUMP00000116009
    • OTTHUMP00000229510
    • OTTHUMP00000229511
    • OTTHUMP00000229512
    • OTTHUMP00000229514
    • OTTHUMP00000229515
    • OTTHUMP00000229516
    • Preprocathepsin B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse Cathepsin B ELISA Kit images

  • Representative Standard Curve Using ab119585.

Protocols

References for Mouse Cathepsin B ELISA Kit (ab119585)

ab119585 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
I was interested in detecting secreted cathepsin B in rat hippocampal tissue. Although the kit uses antibodies designed against mouse, mouse and rat cathepsin B share >90% homology. I was able to successfully detect cathepsin B in the supernatant from organotypic hippocampal slices (D.I.V. 10-14) cultured from Wistar rats (P7). The assay was sensitive, showing a positive signal when tested with 200uL of serum-free media, which was washed over the slice for 10 minutes prior to processing. The assay also showed low well-to-well variability when identical samples were run on multiple wells. Serum-free culture media showed little or no reactivity.
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Mr. Zahid Padamsey

Verified customer

Submitted Aug 11 2015

Thank you for contacting Abcam.

I do apologize for the delay in returning this information to you, especially as I image you have already found the results from the mistaken mixing ofthe biotinylation solution with sample buffer instead of a...

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