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|Sample type||Average %||Range|
|Cell culture supernatant||101.34||91% - 110%|
|Serum||96.78||87% - 108%|
|Plasma||95.79||85% - 107%|
Abcam’s CD153 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD153 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CD153 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD153 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD153 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD153 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|100X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Mouse CD153||2 vials|
|CD153 Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Mouse CD153 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100672 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100672 has not yet been referenced specifically in any publications.