Mouse CD153 ELISA Kit (ab100672)
- Datasheet
- Specific References
- Protocols
Overview
- Product nameMouse CD153 ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity< 0.5 pg/ml
- Range0.41 pg/ml - 100 pg/ml
- Recovery
99 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 101.34 91% - 110% Serum 96.78 87% - 108% Plasma 95.79 85% - 107% - Assay durationMultiple steps standard assay
- Species reactivityReacts with: Mouse
- Product overview
Abcam’s CD153 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD153 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CD153 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD153 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD153 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD153 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsSuitable for: Sandwich ELISAmore details
- PlatformMicroplate
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CD153 2 vials CD153 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CD153 Standard 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml - Research areas
- RelevanceCD153, also known as (CD30 ligand), is a 40KD type II transmembrane protein belonging to the TNF superfamily. In mouse CD153 is expressed primarily on activated CD4+ve T cells. The interaction of CD153 with its ligand CD30 is reported to enhance the proliferation of anti CD3/CD28 costimulated T cells.
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Alternative names
- CD153 antigen
- CD30 antigen ligand
- CD30 L
see all -
Database links
- Entrez Gene: 21949 Mouse
- SwissProt: P32972 Mouse
Applications
Our Abpromise guarantee covers the use of ab100672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Mouse CD153 ELISA Kit images
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Typical Standard CurveRepresentative Standard Curve using ab100672
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Typical Standard CurveRepresentative Standard Curve using ab100672
Protocols
References for Mouse CD153 ELISA Kit (ab100672)
ab100672 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"