Overview

  • Product name
    Mouse CD153 ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.5 pg/ml
  • Range
    0.41 pg/ml - 100 pg/ml
  • Recovery

    99 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.34 91% - 110%
    Serum 96.78 87% - 108%
    Plasma 95.79 85% - 107%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s CD153 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD153 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse CD153 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD153 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD153 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD153 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse CD153 2 vials
    CD153 Microplate (12 strips x 8 wells) 1 unit
    Recombinant Mouse CD153 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    CD153, also known as (CD30 ligand), is a 40KD type II transmembrane protein belonging to the TNF superfamily. In mouse CD153 is expressed primarily on activated CD4+ve T cells. The interaction of CD153 with its ligand CD30 is reported to enhance the proliferation of anti CD3/CD28 costimulated T cells.
  • Alternative names
    • CD153 antigen
    • CD30 antigen ligand
    • CD30 L
    • CD30 ligand
    • CD30L
    • CD30LG
    • MGC138144
    • TNFSF8
    • Tumor necrosis factor (ligand) superfamily member 8
    • Tumor necrosis factor ligand superfamily member 8
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab100672 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100672

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab100672

       

    Protocols

    References

    ab100672 has not yet been referenced specifically in any publications.

    Publishing research using ab100672? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab100672.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up