Overview

  • Product name
    Mouse CD23 Matched Antibody Pair Kit
    See all CD23 kits
  • Detection method
    Colorimetric
  • Assay type
    ELISA set
  • Range
    15.61 pg/ml - 1000 pg/ml
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse CD23. 


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. 


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab206984) which uses the same antibody pair. 


    Buffer information: The capture antibody is glycerol free. The detector antibody contains glycerol.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications
    Suitable for: ELISAmore details
  • Platform
    Reagents

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse CD23 Capture Antibody 1 x 100µg 1 x 20µg
    Mouse CD23 Detector Antibody 1 x 25µg 1 x 5µg
    Mouse CD23 Lyophilized Protein 1 vial 1 vial
  • Research areas
  • Function
    This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane. Secreted. Also exists as a soluble excreted form.
  • Information by UniProt
  • Alternative names
    • Blast 2
    • BLAST-2
    • Blast2
    • C type lectin domain family 4 member J
    • C-type lectin domain family 4 member J
    • C-type lectin domain family 4, member J
    • CD 23
    • CD 23A
    • CD23
    • CD23 antigen
    • CD23A
    • CLEC 4J
    • CLEC4J
    • Fc epsilon receptor II
    • Fc epsilon RII
    • Fc fragment of IgE low affinity II receptor for
    • Fc fragment of IgE receptor II
    • Fc fragment of IgE, low affinity II, receptor for (CD23)
    • Fc of IgE, low affinity II, receptor for (CD23)
    • Fc receptor IgE low affinity II alpha polypeptide
    • Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a
    • Fc-epsilon-RII
    • FCE 2
    • FCE2
    • FCER 2
    • Fcer2
    • FCER2_HUMAN
    • FCER2A
    • FceRII
    • IgE binding factor
    • IgE receptor lymphocyte
    • IgE-binding factor
    • IGEBF
    • Immunoglobulin E binding factor
    • Immunoglobulin E receptor, low affinity II
    • Immunoglobulin E-binding factor
    • Immunoglobulin epsilon chain
    • LEUKOCYTE ANTIGEN CD23
    • Low Affinity IgE Receptor
    • Low affinity immunoglobulin epsilon Fc receptor
    • Low affinity immunoglobulin epsilon Fc receptor membrane bound form
    • Low affinity immunoglobulin epsilon Fc receptor soluble form
    • Ly-42
    • Ly42
    • Lymphocyte antigen CD23
    • Lymphocyte IgE receptor
    • MGC93219
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab216065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab216065 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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