Overview

  • Product name
    Mouse CD62P ELISA Kit (P-Selectin)
    See all CD62P kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.62 83% - 105%
    Serum 92.38 80% - 102%
    Plasma 93.57 81% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s P-Selectin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse P-Selectin in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse P-Selectin coated on a 96-well plate. Standards and samples are pipetted into the wells and P-Selectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse P-Selectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of P-Selectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Mouse P-Selectin ELISA Kit (CD62P) (ab200014) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse P-Selectin 2 vials
    P-Selectin Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse P-Selectin Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • Tissue specificity
    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • Involvement in disease
    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Alternative names
    • Antigen CD62
    • CD62
    • CD62 antigen like family member P
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP 140
    • GMP-140
    • GMP140
    • Granule membrane protein 140
    • Granule membrane protein 140kDa
    • Granulocyte membrane protein
    • GRMP
    • LECAM3
    • Leukocyte endothelial cell adhesion molecule 3
    • Leukocyte-endothelial cell adhesion molecule 3
    • LYAM3_HUMAN
    • P selectin
    • P-selectin
    • PADGEM
    • Platelet activation dependent granule external membrane protein
    • Platelet activation dependent granule-external membrane protein
    • Platelet alpha granule membrane protein
    • PSEL
    • Selectin P
    • Selectin P (granule membrane protein 140kDa antigen CD62)
    • SELP
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab100737

  • Representative standard curve using ab100737

Protocols

References

ab100737 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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