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|Sample type||Average %||Range|
|Serum||133.4||127% - 140%|
|Plasma||128.5||112% - 140%|
|Cell culture media||132||121% - 147%|
Abcam’s Complement C5a mouse ELISA Kit (ab193718) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Complement C5a in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse Complement C5a coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Complement C5a present in the samples. The wells are washed and biotinylated anti-mouse Complement C5a antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Complement C5a bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated mouse C5a detection antibody (lyophilized)||2 vials|
|mouse C5a Standards||2 vials|
|Pre-coated mouse C5a Microplate (12 strips x 8 wells)||1 unit|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab193718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab193718 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"