Mouse CT-1 ELISA Kit (ab155453)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.25 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CT-1 ELISA Kit
See all CT-1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.2 ng/ml -
Range
0.25 ng/ml - 60 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 78.13 70% - 88% Plasma 83.28 73% - 96% Cell culture media 92.96 84% - 100% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam's CT-1 Mouse ELISA Kit (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Mouse CT-1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for CT-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CT-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CT-1 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CT-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse CT-1 2 vials CT-1 Microplate (12 x 8 wells) 1 unit Recombinant Mouse CT-1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. -
Tissue specificity
Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cardiophin 1
- Cardiophin1
- Cardiotrophin I
see all -
Database links
- Entrez Gene: 13019 Mouse
- SwissProt: Q60753 Mouse
- Unigene: 389954 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155453 has not yet been referenced specifically in any publications.