Overview

  • Product name
    Mouse CXCL15 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.8 ng/ml
  • Range
    0.8 ng/ml - 200 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 120.1 102% - 128%
    Plasma 134 118% - 150%
    Cell culture media 135.5 124% - 144%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s CXCL15 mouse ELISA Kit (ab193720) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL15 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse CXCL15 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CXCL15 present in the samples. The wells are washed and biotinylated anti-mouse CXCL15 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CXCL15 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent D 1 x 15ml
    800X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Biotinylated mouse CXCL15 detection antibody (lyophilized) 2 vials
    mouse CXCL15 Standards 2 vials
    Pre-coated mouse CXCL15 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function
    Chemotactic for neutrophils. Involved in lung-specific neutrophil trafficking during normal and inflammatory conditions.
  • Tissue specificity
    Expression restricted to the lung, produced by bronchoepithelial cells and is released into the airways. Expressed at low levels in fetal lung.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • C-X-C motif chemokine 15
    • Chemokine (C-X-C motif) ligand 15
    • Cxcl15
    • CXL15_MOUSE
    • Il8
    • Interleukin 8
    • Lungkine
    • Scyb15
    • Small inducible cytokine subfamily B member 15
    • Small-inducible cytokine B15
    • weche
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve using ab193720 CXCL15 Mouse ELISA Kit.

Protocols

References

ab193720 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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