• Product name
    Mouse CXCL5 ELISA Kit (GCP-2)
    See all CXCL5 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 35 pg/ml
  • Range
    31.25 pg/ml - 2000 pg/ml
  • Recovery

    91 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 83.94 74% - 94%
    Serum 98.05 86% - 106%
    Plasma 93.11 83% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s CXCL5 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CXCL5 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for CXCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Mouse CXCL5 (lyophilized) 2 vials
    CXCL5 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse CXCL5 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
  • Alternative names
    • AMCF II
    • AMCFII
    • C X C motif chemokine 5
    • Chemokine (C X C motif) ligand 5
    • CXCL 5
    • ENA 78
    • ENA78
    • Epithelial derived neutrophil activating protein 78
    • GCP 2
    • LIX
    • Neutrophil activating peptide ENA 78
    • Neutrophil activating protein 78
    • SCYB5
    • Scyb6
    • Small inducible cytokine B5
    • Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78)
    • Small inducible cytokine subfamily B, member 15
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100719



This product has been referenced in:
  • Lee CH  et al. Glutamine suppresses airway neutrophilia by blocking cytosolic phospholipase A(2) via an induction of MAPK phosphatase-1. J Immunol 189:5139-46 (2012). Mouse . Read more (PubMed: 23109722) »

See 1 Publication for this product

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