Mouse Cytokine Antibody Array - Membrane (144 Targets) (ab193660)

Overview

  • Product name
    Mouse Cytokine Antibody Array - Membrane (144 Targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab193660 can be used for the simultaneous detection of 144 mouse cytokine proteins in serum, plasma, cell culture media and other liquid samples types. It comes as 1 kit including 3 separate membranes labelled C3, C4 and C5 (see below for images showing the array layout on each membrane). 


    Target Names: 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), ACE, ALK-1, Amphiregulin, Axl, bFGF, BLC (CXCL13), Cardiotrophin-1 (CT-1), CD27 (TNFRSF7), CD27 Ligand (TNFSF7), CD30 Ligand (TNFSF8), CD30 (TNFRSF8), CD36 (SR-B3), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), Chordin, CRG-2, CTACK (CCL27), CTLA-4 (CD152), CXCL16, Decorin, DKK-1, CD26 (DPPIV), Dtk, E-Cadherin, EGF, Endoglin CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Epigen, Epiregulin, E-Selectin, Fas Ligand (TNFSF6), Fc gamma RIIB (CD32b), Flt-3 Ligand, Fractalkine (CX3CL1), Galectin-1, GCSF, GITR TNFRSF18), GITR Ligand (TNFSF18), GM-CSF, Granzyme B, Gas 1, Gas 6, HAI-1, HGF, HGFR, ICAM-1 (CD54), IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IGF-1, IGF-2, IL-1 beta (IL-1 F2), IL-1 R4 (ST2), IL-10, IL-11, IL-12 p40/p70, IL-12 p70, IL-13, IL-15, IL-17A, IL-17B, IL-17 RB, IL-17E (IL-25), IL-17F, IL-1 alpha (IL-1 F1), IL-1 RA (IL-1 F3), IL-2, IL-2 R alpha, IL-20, IL-21, IL-28A (IFN-LAMBDA 2), IL-3, IL-3 R beta, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-9, I-TAC (CXCL11), JAM-A (CD321/F11R), KC (CXCL1), Leptin, Leptin R, LIX, L-Selectin (CD62L), Lungkine (CXCL15), Lymphotactin (XCL1), MAdCAM-1, MCP-1, MCP-5, M-CSF, MDC, MFG-E8, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 gamma, MIP-2, MIP-3 beta (CCL19), MIP-3 alpha (CCL20), MMP-2, MMP-3, Neprilysin, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), Pentraxin-3 (TSG-14), Platelet Factor 4 (CXCL4), Prolactin, Pro-MMP-9, P-Selectin, RAGE, RANTES (CCL5), Resistin, SCF, SDF-1 alpha (CXCL12 alpha), Sonic Hedgehog N-Terminal (Shh-N), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TACI (TNFRSF13B), TARC (CCL17), I-309 (TCA-3/CCL1), TECK (CCL25), TCK-1 (CXCL7), TIMP-1, TIMP-2, TNF alpha, Thrombopoietin (TPO), TRANCE (TNFSF11), TREM-1, TROY (TNFRSF19), TSLP, TWEAK (TNFSF12), TWEAK R (TNFRSF12), VCAM-1 (CD106), VEGF-A, VEGFR1, VEGFR2, VEGFR3, VEGF-D


    Learn more about membrane antibody arrays

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 2 x 50µl 4 x 50µl 1 x 50µl
    1X Blocking Buffer 3 x 25ml 8 x 25ml 2 x 25ml
    20X Wash Buffer I 2 x 20ml 4 x 20ml 1 x 20ml
    20X Wash Buffer II 2 x 20ml 4 x 20ml 1 x 20ml
    2X Cell Lysis Buffer 2 x 16ml 4 x 16ml 1 x 16ml
    8-Well Incubation Tray (with Lid) 2 units 4 units 1 unit
    Biotinylated Antibody Cocktail (C3) 2 vials 4 vials 1 vial
    Biotinylated Antibody Cocktail (C4) 2 vials 4 vials 1 vial
    Biotinylated Antibody Cocktail (C9) 2 vials 4 vials 1 vial
    Detection Buffer C 2 x 2.5ml 4 x 2.5ml 1 x 2.5ml
    Detection Buffer D 2 x 2.5ml 4 x 2.5ml 1 x 2.5ml
    Mouse Cytokine Antibody Arrays Membranes (C3) 1 x 4 units 1 x 8 units 1 x 2 units
    Mouse Cytokine Antibody Arrays Membranes (C4) 1 x 4 units 1 x 8 units 1 x 2 units
    Mouse Cytokine Antibody Arrays Membranes (C5) 1 x 4 units 1 x 8 units 1 x 2 units
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical images obtained with Abcam Mouse Cytokine Antibody Array - Membrane (144 Targets) ab193660. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at room temperature for 1 minute.

Protocols

References

ab193660 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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