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Abcam Mouse Cytokine Antibody Array - Membrane (ab169820) detects 97 Mouse Cytokines. Suitable for all liquid sample types.
Targets: 6Ckine, ALK-1, Amphiregulin, Axl, BLC, Cardiotrophin-1, CD27, CD27 Ligand, CD30, CD30 Ligand, CD36/SR-B3, CTLA-4, CXCL16, Decorin, Dkk-1, E-Cadherin, EGF, Eotaxin-1, Eotaxin-2, Epigen, E-Selectin, Fas Ligand, Fc gamma RIIB, Flt-3 Ligand, Fractalkine, Galectin-1, Gas 6, G-CSF, GITR, GITR Ligand, Granzyme B, HAI-1, HGF, IFN-gamma, IGFBP-5, IGFBP-6, IGF-2, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-17, IL-17B R, IL-17E, IL-17F, IL-20, IL-21, I-TAC, JAM-A, KC/CXCL1, Leptin/OB, Leptin R, L-Selectin, Lungkine, MadCAM-1, MCP-1, MDC, MFG-E8, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-2, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, RANTES, SCF, sTNFRI, sTNFRII, TACI, TARC, TNF alpha, Thrombopoietin, TRANCE, TROY, TWEAK R, VCAM-1, VEGF-A, VEGFR1, VEGFR3, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
|Components||1 x 4 Membranes||1 x 8 Membranes||1 x 2 Membranes|
|1,000X HRP-Streptavidin Buffer||1 x 50µl||2 x 50µl||1 x 50µl|
|Blocking Buffer||2 x 25ml||4 x 25ml||1 x 25ml|
|20X Wash Buffer I||1 x 20ml||2 x 20ml||2 x 10ml|
|20X Wash Buffer II||1 x 20ml||2 x 20ml||1 x 10ml|
|2X Cell Lysis Buffer||1 x 16ml||2 x 16ml||1 x 10ml|
|4000X Biotin-Conjugated Anti Cytokines||4 vials||8 vials||2 vials|
|4-Well Plastic Tray||1 unit||2 units||1 unit|
|Cytokine Antibody Array Membranes||4 units||8 units||2 units|
|Detection Buffer C||1 x 2.5ml||2 x 2.5ml||1 x 1.5ml|
|Detection Buffer D||1 x 2.5ml||2 x 2.5ml||1 x 1.5ml|
Our Abpromise guarantee covers the use of ab169820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Multiplex Protein Detection||Use at an assay dependent concentration.|
ab169820 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"