Mouse Cytokine Antibody Array - Membrane (97 targets) (ab169820)

Overview

  • Product name
    Mouse Cytokine Antibody Array - Membrane (97 targets)
    See all Cytokine Antibody Array antibody arrays
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay type
    Semi-quantitative
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam Mouse Cytokine Antibody Array - Membrane (ab169820) detects 97 Mouse Cytokines. Suitable for all liquid sample types. 


    Targets: 6Ckine, ALK-1, Amphiregulin, Axl, BLC, Cardiotrophin-1, CD27, CD27 Ligand, CD30, CD30 Ligand, CD36/SR-B3, CTLA-4, CXCL16, Decorin, Dkk-1, E-Cadherin, EGF, Eotaxin-1, Eotaxin-2, Epigen, E-Selectin, Fas Ligand, Fc gamma RIIB, Flt-3 Ligand, Fractalkine, Galectin-1, Gas 6, G-CSF, GITR, GITR Ligand, Granzyme B, HAI-1, HGF, IFN-gamma, IGFBP-5, IGFBP-6, IGF-2, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-17, IL-17B R, IL-17E, IL-17F, IL-20, IL-21, I-TAC, JAM-A, KC/CXCL1, Leptin/OB, Leptin R, L-Selectin, Lungkine, MadCAM-1, MCP-1, MDC, MFG-E8, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-2, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, RANTES, SCF, sTNFRI, sTNFRII, TACI, TARC, TNF alpha, Thrombopoietin, TRANCE, TROY, TWEAK R, VCAM-1, VEGF-A, VEGFR1, VEGFR3, VEGF-D


    Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.


    Learn more about membrane antibody arrays


     

  • Notes

    If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

  • Tested applications
    Suitable for: Multiplex Protein Detectionmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes
    1,000X HRP-Streptavidin Buffer 1 x 50µl 2 x 50µl 1 x 50µl
    Blocking Buffer 2 x 25ml 4 x 25ml 1 x 25ml
    20X Wash Buffer I 1 x 20ml 2 x 20ml 2 x 10ml
    20X Wash Buffer II 1 x 20ml 2 x 20ml 1 x 10ml
    2X Cell Lysis Buffer 1 x 16ml 2 x 16ml 1 x 10ml
    4000X Biotin-Conjugated Anti Cytokines 4 vials 8 vials 2 vials
    4-Well Plastic Tray 1 unit 2 units 1 unit
    Cytokine Antibody Array Membranes 4 units 8 units 2 units
    Detection Buffer C 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml
    Detection Buffer D 1 x 2.5ml 2 x 2.5ml 1 x 1.5ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Multiplex Protein Detection Use at an assay dependent concentration.

Images

  • Typical results obtained with Abcam Membrane Antibody Arrays. The membrane was probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.

Protocols

References

ab169820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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