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|Sample type||Average %||Range|
|Cell culture supernatant||79.55||71% - 85%|
|Plasma||91.38||83% - 100%|
Abcam’s DKK1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DKK1 in plasma and cell culture supernatants. (Mouse DKK1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for mouse DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Mouse DKK1 in normal serum with ab113347, only in serum spiked with Mouse DKK1.
Get better reproducibility in only 90 minutes with Mouse DKK1 ELISA Kit (ab197746) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-mouse DKK1||2 vials|
|DKK1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse DKK1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab113347 has not yet been referenced specifically in any publications.