Overview

  • Product name

    Mouse DKK1 ELISA Kit (Dickkopf-1)


    See all DKK1 kits
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 79.55 71% - 85%
    Plasma 91.38 83% - 100%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s DKK1 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DKK1 in plasma and cell culture supernatants. (Mouse DKK1 concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for mouse DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Mouse DKK1 in normal serum with ab113347, only in serum spiked with Mouse DKK1.


    Get better reproducibility in only 90 minutes with Mouse DKK1 ELISA Kit (ab197746) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceDKK1 is an inhibitor of the Wnt signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
  • Alternative names
    • Dickkopf 1
    • Dickkopf homolog 1
    • Dickkopf related protein 1
    • DKK 1
    • hDkk 1
    • hDkk1
    • mDkk1
    • SK
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab113347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse DKK1 ELISA Kit (Dickkopf-1)

images

  • Representative standard curve using ab113347

  • Representative standard curve using ab113347

Protocols

References for

Mouse DKK1 ELISA Kit (Dickkopf-1)

(ab113347)

ab113347 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"