Overview

  • Product name
    Mouse Eotaxin-2 ELISA Kit (CCL24)
    See all Eotaxin 2 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    2.46 pg/ml - 600 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.65 89% - 102%
    Serum 103.1 93% - 112%
    Plasma 113.1 79% - 137%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Eotaxin-2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Eotaxin-2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse Eotaxin-2 coated on a 96- well plate. Standards and samples are pipetted into the wells and Eotaxin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Eotaxin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Mouse Eotaxin-2 2 vials
    Eotaxin-2 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse Eotaxin-2 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.
  • Cellular localization
    Secreted
  • Alternative names
    • C C motif chemokine 24
    • CCL24
    • Chemokine (C C motif) ligand 24
    • Chemokine CC Motif Ligand 2
    • CK beta 6
    • Ckb 6
    • Ckb6
    • Eosinophil chemotactic protein 2
    • Eotaxin 2 like protein
    • MPIF 2
    • MPIF2
    • Myeloid progenitor inhibitory factor 2
    • OTTHUMP00000210225
    • SCYA24
    • Small inducible cytokine A24
    • Small inducible cytokine subfamily A (Cys Cys) member 24
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100681

  • Representative Standard Curve using ab100681

Protocols

References

This product has been referenced in:
  • Cheng M  et al. Commensal microbiota maintains alveolar macrophages with a low level of CCL24 production to generate anti-metastatic tumor activity. Sci Rep 7:7471 (2017). Read more (PubMed: 28785009) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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