Mouse Epiregulin ELISA Kit (EREG) (ab100682)

Overview

  • Product name
    Mouse Epiregulin ELISA Kit (EREG)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.6 94% - 110%
    Plasma 73.94 67% - 90%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Product overview

    Abcam’s Epiregulin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Epiregulin in plasma and cell culture supernatants.


    This assay employs an antibody specific for Mouse Epiregulin coated on a 96- well plate. Standards and samples are pipetted into the wells and Epiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Epiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Epiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Mouse Epiregulin in normal serum with ab100682, only in serum spiked with Mouse Epiregulin.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100682

  • Representative Standard Curve using ab100682

Protocols

References

ab100682 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

The Epiregulin kit ab100682 is not suitable for Human samples since one of the antibody has shown no cross-reactivity with Human epiregulin, and unfortunately we do not have an ELISA kit for Human Epiregulin in our...

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Thank you very much for your call this week and for your patience while I have been in touch with the lab regarding your enquiry.

The capture antibodyin this kit does cross-react with the human epiregulin, however the detection antibodyis not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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