Overview

  • Product name
    Mouse Factor D ELISA Kit
    See all Factor D kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.03 ng/ml
  • Range
    0.031 ng/ml - 1 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Factor D Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor D in cell culture supernatants, urine, plasma and serum.

    A Factor D specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor D specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor D captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Mouse Factor D Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Factor D Microplate (12 x 8 well strips) 1 unit
    Factor D Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • Involvement in disease
    Complement factor D deficiency (CFDD) [MIM:613912]: An immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Adipsin
    • ADN
    • C3 convertase activator
    • CFAD_HUMAN
    • CFD
    • Complement factor D
    • Complement factor D preproprotein
    • D component of complement
    • DF
    • PFD
    • Properdin factor D
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab170244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab170244

Protocols

References

ab170244 has not yet been referenced specifically in any publications.

Customer reviews and Q&As



I confirm the source of the antigen used in this kit is E.coli.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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