• Product nameMouse Galectin 3 ELISA Kit
    See all Galectin 3 kits
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.19 pg/ml - 2000 pg/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124.3 116% - 131%
    Serum 111.8 101% - 120%
    Plasma 99.08 90% - 107%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Galectin 3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Galectin 3 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse Galectin 3 coated on a 96- well plate. Standards and samples are pipetted into the wells and Galectin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Galectin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceGalactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis By similarity. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Probably forms homo- or heterodimers. Interacts with DMBT1. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Contains 1 galectin domain.
  • Cellular localizationCytoplasmic, Nuclear and Secreted
  • Alternative names
    • 35 kDa lectin
    • Carbohydrate-binding protein 35
    • CBP 35
    • Gal-3
    • Galactose-specific lectin 3
    • Galactoside-binding protein
    • GALBP
    • Galectin-3
    • GALIG
    • IgE-binding protein
    • L-31
    • Laminin-binding protein
    • Lectin L-29
    • lectin, galactoside-binding, soluble, 3
    • LGALS2
    • Mac-2 antigen
    • MAC2
    • Macrophage galactose-specific lectin
    see all
  • Database links


Our Abpromise guarantee covers the use of ab113343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse Galectin 3 ELISA Kit images

  • Representative Standard Curve using ab113343

  • Representative Standard Curve using ab113343


References for Mouse Galectin 3 ELISA Kit (ab113343)

ab113343 has not yet been referenced specifically in any publications.

Product Wall

While this ELISA kit was neither designed nor validated with tissue lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. If you decide to test these kits with tissue lysate samples, we recom...

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Thank you for the clarification. This kit shows 10% reactivity with human. Please let me know if you have any further questions.