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|Sample type||Average %||Range|
|Cell culture supernatant||124.3||116% - 131%|
|Serum||111.8||101% - 120%|
|Plasma||99.08||90% - 107%|
Abcam’s Galectin 3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Galectin 3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse Galectin 3 coated on a 96- well plate. Standards and samples are pipetted into the wells and Galectin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Galectin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Galectin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Mouse Galectin 3||2 vials|
|Galectin 3 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse Galectin 3 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab113343 has not yet been referenced specifically in any publications.