Overview

  • Product nameMouse GM-CSF ELISA Kit
    See all GM-CSF kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 279.2pg/ml 14.7 5.2%
    B 8 62.5pg/ml 2.52 4%
    Inter-assay
    Sample n Mean SD CV%
    A 7 271.9pg/ml 10.8 3.9%
    B 7 65.4pg/ml 4.3 6.5%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 9 pg/ml
  • Range
    15.6 pg/ml - 500 pg/ml
  • Assay time
    3h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s GM-CSF mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of GM-CSF in mouse serum, buffered solutions and cell culture medium.

    A monoclonal antibody specific for GM-CSF has been coated onto the wells of the microtiter strips provided. Samples, including standards of known GM-CSF concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for GM-CSF are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of GM-CSF present in the samples.

    This kit will recognize both endogenous and recombinant mouse GM-CSF.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similaritiesBelongs to the GM-CSF family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab46078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse GM-CSF ELISA Kit images

  • Representative Standard Curve using ab46078

Protocols

References for Mouse GM-CSF ELISA Kit (ab46078)

ab46078 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab46078.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"