• Product nameMouse GM-CSF ELISA Kit (CSF2)
    See all GM-CSF kits
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    99 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.53 88% - 108%
    Plasma 100.32 89% - 107%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s GM-CSF Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse GM-CSF in plasma and cell culture supernatants.

    This assay employs an antibody specific for mouse GM-CSF coated on a 96- well plate. Standards and samples are pipetted into the wells and GM-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Mouse GM-CSF in normal serum with ab100685, only in serum spiked with Mouse GM-CSF.

    Get higher sensitivity in only 90 minutes with Mouse GM-CSF ELISA Kit (CSF2) (ab201276) from our SimpleStep ELISA® range.


  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionCytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • Sequence similaritiesBelongs to the GM-CSF family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab100685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse GM-CSF ELISA Kit (CSF2) images

  • Representative Standard Curve using ab100685

  • Representative Standard Curve using ab100685


References for Mouse GM-CSF ELISA Kit (CSF2) (ab100685)

ab100685 has not yet been referenced specifically in any publications.

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