Mouse heart tissue lysate - total protein (14 days) (ab7194)

Overview

  • Product name
    Mouse heart tissue lysate - total protein (14 days)
    See all Heart lysates
  • General notes
    Source: Outbred NIH-Swiss Albino mice (14 days old)


    The mouse heart tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applications
    Suitable for: WBmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab7194 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Ready to load; 10-20 µg/lane is recommended for mini gel.

    References for Mouse heart tissue lysate - total protein (14 days) (ab7194)

    ab7194 has not yet been referenced specifically in any publications.

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