Mouse heart tissue lysate - total protein (7 days) (ab7196)

Overview

  • Product nameMouse heart tissue lysate - total protein (7 days)
    See all Heart lysates
  • Description
    Mouse heart total protein lysate
  • General notesSource: Outbred NIH-Swiss Albino mice (7 days old)


    The mouse heart tissue was collected from seven (7) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applicationsSuitable for: WBmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab7196 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. 10 µg to 20 µg per lane is recommended for mini gel.

    References for Mouse heart tissue lysate - total protein (7 days) (ab7196)

    ab7196 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Questions for ab7196.
    Please use the link above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"