• Product nameMouse IGFBP2 ELISA Kit
    See all IGFBP2 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.6 ng/ml
  • Range
    0.823 ng/ml - 600 ng/ml
  • Recovery

    91 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 112.5 103% - 122%
    Serum 81.45 72% - 90%
    Plasma 80.22 73% - 92%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s IGFBP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Mouse IGFBP2 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionInhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • Sequence similaritiesContains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • DomainThe C-terminus is required for IGF-binding and growth inhibition.
  • Post-translational
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • BP 2
    • BP2
    • IBP 2
    • IBP-2
    • IBP2
    • IBP2_HUMAN
    • IGF binding protein 2
    • IGF BP53
    • IGF-binding protein 2
    • IGF-BP53
    • IGFBP-2
    • IGFBP2
    • IGFBP53
    • Insulin Like Growth Factor Binding Protein 2
    • Insulin like growth factor binding protein 2 36kDa
    • Insulin like growth factor-binding protein 2 precursor
    • Insulin-like growth factor-binding protein 2
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Mouse IGFBP2 ELISA Kit images


References for Mouse IGFBP2 ELISA Kit (ab100691)

This product has been referenced in:
  • Moya-Pérez A  et al. Hepatic molecular responses to Bifidobacterium pseudocatenulatum CECT 7765 in a mouse model of diet-induced obesity. Nutr Metab Cardiovasc Dis N/A:N/A (2013). Read more (PubMed: 23831006) »

See 1 Publication for this product

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Thank you for your inquiry.

I heard back from the lab that the suggested dilution for normal serum/plasma is 2 - 10 fold. They do note that levels of the target protein may vary between different specimens. Optimal dilution factors for each s...

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Sie können das Assay Diluent Bnun unter ab125050bestellen.

Leider verkaufen wir die einzelnen Kit Komponenten nicht separat, so dass ich Ihnen leider in Ihrem Fall nicht weiterhelfen kann.