|Sample type||Average %||Range|
|Cell culture supernatant||112.5||103% - 122%|
|Serum||81.45||72% - 90%|
|Plasma||80.22||73% - 92%|
Abcam’s IGFBP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IGFBP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse IGFBP2 coated on a 96- well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Mouse IGFBP2||2 vials|
|IGFBP2 Microplate (12 x 8 wells)||1 unit|
|Recombinant Mouse IGFBP2 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|